ntributions NM, GJD, HSO, and JGB created and planned the investigation. MH prepared fungal cultures. CB and SS prepared ADAM8 web activitybased probes employed in this study. NM collected secretome samples and performed activitybased protein profil ing experiments. NM collected and analysed proteomic information. DN performed bioinformatic analysis. NM and MS ready P. pastoris strains, made and purified recombinant enzymes, and performed activity assays. NM wrote the manuscript with input from each of the authors. All authors study and approved the final manuscript. Funding The authors thank the All-natural Sciences and Engineering Research Council of Canada (PostDoctoral Fellowship to NGSM), the Royal Society (Ken Murray Investigation Professorship to GJD), the Biotechnology and Biological Sciences Investigation Council (BBSRC) (grant BB/R001162/1 to GJD), the French National Analysis Agency (ANR13BIME0002 to JGB), the Netherlands Organization for Scientific Investigation (NWO Top rated grant 2018714.018.002 to HSO), along with the European Investigation Council (ERC2011AdG290836 “Chembiosphing” to HSO, ERC2020SyG951231 “Carbocentre” to GJD and HSO). Proteomics data have been collected in the York Centre of Excellence in Mass Spectrometry, which was developed thanks to a significant capital investment through Science City York, sup ported by Yorkshire Forward with funds from the Northern Way Initiative, and subsequent help from EPSRC (EP/K039660/1; EP/M028127/1). Availability of data and components Pichia pastoris strains and samples of recombinant proteins could be offered from Gideon Davies ([email protected]). Samples of ABPCel, ABPXyl, and ABPGlc may be readily available from D3 Receptor Species Herman Overkleeft (h.s.overkleeft@lic. leidenuniv.nl). Basidiomycete fungi are readily available in the fungal culture collection of your International Centre of Microbial Sources (CIRMCF) at the French National Institute for Agricultural investigation (INRA; Marseille, France). Genome sequences for each in the fungi utilized in this study are offered from Mycocosm (mycocosm.jgi.doe.gov/mycocosm/home) (DOE Joint Genome Institute, Walnut Creek, California). Other datasets made use of and/or ana lysed throughout the current study are obtainable in the corresponding author on affordable request.Author details 1 York Structural Biology Laboratory, Department of Chemistry, The University of York, Heslington YO10 5DD, York, UK. two Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. 3 UMR1163 Bio diversitet Biotechnologie Fongiques, Facultdes Sciences de Luminy, INRAE, Aix Marseille Univ, 13288 Marseille, France. four Polytech Marseille, Aix Marseille Univ, 13288 Marseille, France. Received: 8 October 2021 Accepted: 6 JanuaryDeclarationsEthics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no competing interests.References 1. Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;two(61):2639. 2. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;3(two):210. 3. Celiska E, Nicaud JM, Bialas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide sources: overview on starch, cellulose, xylan, and inulin. Appl Microbiol Biotechnol. 2021;105(three):9759. 4. Schlembach I, Hosseinpour Tehrani H, Blank LM, B hs J, Wierckx N, Regestein L, et al. Consolidate
