Was dependent on the presence of functional viral Env machinery,ISEV2019 ABSTRACT BOOKeither from actively circulating viruses which includes VSV-G, T-type calcium channel Gene ID rabies, influenza, and mokola viruses or from human endogenous retroviruses (HERVs) Env proteins for example syncytin-1. Summary/Conclusion: EVs developed in the absence of viral Env machinery are poorly fusogenic and are unlikely to be effective mediators of cell-tocell communication by way of the delivery of EV contents for the cytoplasm. In contrast, viral Env proteins substantially enhance EV fusogenicity, suggesting that EV fusion and communication may perhaps take place and play a substantial part through viral infections. Furthermore, cells expressing the HERV Env syncytin-1 including lots of human cancers also give rise to fusogenic EVs that may contribute to tumour establishment, development, and metastasis. These findings recommend that blocking syncytin-mediated EV fusion may be an effective method to block EV communication in human cancers.OS24.Preferential accumulation of copper-free click chemistry-modified exosomes to own pancreatic xenograft in vivo Lizhou Xua, Revadee Liam-Orb, Farid N. Faruqub, Omar Abedc, Danyang Lib, Julie Wangb and Khuloud Al-Jamalba School of Cancer and Pharmaceutical Sciences, King’s College London, London, UK; bKing’s College London, London, UK; cKing’s College London, London, UKResults: Cellular uptake of Exo was time- and dosedependent profiles. Pc derived PANC-1 Exo showed substantially larger and not saturable uptake in PANC1 cells in comparison to B16-F10 Exo (cancer-derived) and HEK-293 Exo (non-cancer derived) which showed decrease and saturable uptake profile at 24 h. In vivo biodistribution studies of PANC-1 Exo in subcutaneous Computer xenograft further confirmed that PANC-1 Exo favoured accumulation in Computer tumours over melanoma (B16-F10) tumours. Summary/Conclusion: A simple and extremely efficient surface modification method through click chemistry was created enabling both in vitro and in vivo tracking of Exo. DoE modelling predicted Pc cells’ preference to PC-derived Exo which was confirmed also in vivo. This Exo dosimetry study could facilitate a rationalized method in Exo-based therapeutics for therapy of cancer in pre-clinical research. Funding: The K. C. Wong Education Foundation plus the Marie Sklodowska-Curie actions, European Commission “Horizon 2020”, EU (H2020-MSCA-IF2016)OS24.TLR4 Accession Certain transfer of hollow gold nanoparticles within exosomes is determined by the exosome origin Maria Sancho-Alberoa, Nuria Navascu b, Gracia Mendozab, Victor Sebastiana, Manuel Arrueboa, Pilar Martin-Duquec and Jesus SantamariaaaIntroduction: Pancreatic cancer (Pc) is one of the deadliest malignancy with couple of productive approaches offered for early diagnosis or therapy. Exosomes (Exo) as one type of extracellular vesicles are currently being investigated as potential theragnostic tools in cancer. Having said that, it is not yet well-understood how Exo are taken up by Pc cells. This operate aims to study the Exo dosimetry and preferential Exo-cell affinity in Computer cells in vitro and in vivo for exploitation of Exo-based delivery of therapeutics. Techniques: Exo are isolated by sucrose cushion ultracentrifugation and characterized for exosomal marker expression, number, purity and shape. Exo were fluorescently labelled by copper-free click chemistry to allow uptake quantification in cells making use of the Design of Experiments (DoE) method. Cellular uptake of Exo was investigated employing flow cytometry and confocal microscopy. Fa.
