Monocytogenes in the processing environment. Certainly, the background RP101988 supplier microbiota is identified
Monocytogenes inside the processing environment. Indeed, the background microbiota is identified to play a role in the protection of pathogens. [25]. In food facilities, Listeria monocytogenes is usually discovered associated with other microorganisms in multispecies biofilms [26,27]. The interactions amongst the microorganisms and Listeria monocytogenes have been shown to modify the capacity from the pathogen to colonize facilities [281], implicating positive effects including resistance to disinfectants and enhanced adherence to surfaces [32] as well as negative effects like nutrient-limiting circumstances and the production of antilisterial compounds [33,34]. A number of research have shown that through the processing of carcasses at the slaughterhouse, a microbiota can survive, come into make contact with with food-contact surfaces which include conveyor belts, detach and therefore contaminate the meals product [11]. In actual fact, inside the case of Listeria monocytogenes, essentially the most frequent pathway of contamination of food products is by cross-contamination with food processing surfaces [29,35,36]. This kind of transfer from the pathogen has been identified because the cause of outbreaks of listeriosis [37]. Biofilms can establish themselves in irregularities around the surface of conveyors and as a result develop into web pages of contamination through the passage of food merchandise [380]Pathogens 2021, ten,three ofIn a recent critique around the microbial diversity and ecology of biofilms linked with L. monocytogenes in the market atmosphere, Fagerlund et al. (2021) highlighted the have to have for higher throughput sequencing (HTS) technology approaches for the detection of interactions among the members of microbial communities present within the biofilms discovered on industrial surfaces. [41]. Many studies have carried out the analysis from the microbiota plus the detection of Listeria monocytogenes for the same surface or sample [424]. However, most of them have chosen classical culture-dependent strategies for the characterization from the microbiota. As a result, the results obtained from these research represent an approximation on the composition from the microbiota given that only a smaller portion of cultivable GNE-371 DNA/RNA Synthesis bacteria are taken into account [45]. Advances in HTS sequencing have resulted in the development of fast and effective techniques for the characterization in the composition of microbial communities. These HTS approaches permit the detection of non-cultivable bacteria at the same time because the detection, as a consequence of their higher sensitivity, of nondominant bacteria. In recent years, few studies have employed HTS technology to attempt the identification with the bacterial genus potentially implicated in the presence of Listeria monocytogenes on surfaces within the food market [28,39,458]. These research successfully identified dominating bacteria identified inside the atmosphere microbiota when Listeria monocytogenes was present or absent. Rodriguez-Lopez et al. (2019) identified the Actinobacteria because the most present taxa within a sample from a meat facility surface [45]. Liu et al. (2016) showed that Pseudomonas psychrophila, Pseudomonas sp., Klebsiella sp., Klebsiella oxytoca and Aeromonas hydrophila were dominant within the microbial neighborhood of Listeria-positive drain samples [46]. A different study from Tan et al. (2019) revealed a distinct microbiota within a facility having a higher occurrence of Listeria monocytogenes [28]. This distinct microbiota was predominated with Pseudomonadaceae plus the fungal household Dipodascaceae. These final results reinforce the concept that the composition.
