Faecalis shown within this study and present within the offered literature migh ously demonstrated and recommend applicability of the oil and TQ against a broad spectrum indicate pathogens. The results of susceptibility extracts and TQ towards the tested substance of bacterialon strain-dependencyconcerning NS seed these bacteria efficiency against [16,19,28,32]. shown in this study and present in the accessible literature may possibly indicate E. coli, E. faecalisThe antibacterial effects of NS seed oil had been observed to become strongly rely ent around the final TQ concentration these samples. on strain-dependency of susceptibilityin the bacteria to the tested substances [16,19,28,32].The antibacterial effects of NS seed oil were observed to become strongly dependent on the final TQ Supplies and Solutions 4. concentration within the samples.four.1. Optimization of Supercritical Carbon Dioxide Polmacoxib Cancer extraction from Nigella Aztreonam Biological Activity sativa Seeds four. Supplies and Procedures 4.1. Optimization of Supercritical Carbon Dioxide Extraction from Nigella sativa Seeds 4.1.1. Plant Material and Extraction Gear four.1.1. Plant Material and Extraction Equipment from FZL firm from Poland, batch num Nigella sativa L. seeds were bought ber Nigella sativa L. seeds were purchased from FZL enterprise from Poland, batch Gdynia, Poland F4568. The Voucher specimen No WLDK/2013 is stored at Biovico, number F4568. The had been ground having a Bosch KM13 is stored (Bosch, Munich, Germany), ten g a The seeds Voucher specimen No WLDK/2013 grinder at Biovico, Gdynia, Poland. The seeds had been ground with a Bosch KM13 grinder (Bosch, Munich, Germany), ten g at after for 2 min. Ground seeds (four.0 g) were put in ten mL stainless steel extraction vessel after for two min. Ground seeds (4.0 g) had been put in 10 mL stainless steel extraction vessels. Supercritical CO2 extraction of NS seed oil was performed with a Waters MV-10 ASF Supercritical CO2 extraction of NS seed oil was performed using a Waters MV-10 ASFE (Waters Corporation, Milford, MA, USA) extractor at distinctive conditions of temperature (Waters Corporation, Milford, MA, USA) extractor at distinct conditions of temperature, stress, extraction time, scCO2 flow (Figure four). CO2 of 99.995 purity purity (AirLiquide stress, extraction time, andand scCO2 flow (Figure four). CO2 of 99.995 (AirLiquide, Cracow, Poland) was applied for extraction oil. Cracow, Poland) was employed for extraction of NSof NS oil.Figure four.four. Flow diagramaof a supercritical carbon dioxide extraction(1. CO2 supply; two.provide; two. coolin Figure Flow diagram of supercritical carbon dioxide extraction program program (1. CO2 cooling heat exchanger; three. 3. CO2 pump; co-solvent vessel; five. co-solvent pump; 6. extraction oven with with ex heat exchanger; CO2 pump; 4. four. co-solvent vessel; five. co-solvent pump; six. extraction oven extraction vessels;7. backpressure regulator; eight. sample collection; 9. wet 9. wet gas meter 2and CO2 release) traction vessels; 7. backpressure regulator; eight. sample collection; gas meter and CO release).four.1.2. Modelling of Oil Extraction in the Nigella sativa SeedsThe evaluation in the effects of stress, temperature, extraction time and scCO2 flow on theTheextraction of the effects of pressure, temperature, extraction time and scCO2 flow oil analysis efficiency from NS seeds was performed applying a central composite on the design and style (CCRD) with response NS seeds was performed The experimental rotatableoil extraction efficiency from surface methodology (RSM).making use of a central composit design and style made use of within this (CCRD) wi.
