Had been cleaned5.5 cm diameter, 7 the flowering period persisted. In the grain size, sediment corers (n = two, from fauna cm and sediment withtaken ahead of, during and immediately after the sexual reproduction period. Sedi of length) have been artificial seawater (30 psu). Then, the sexual spathes contained in each and every corer werewere driedrecording the flowering work. ment corers counted for for 72 h at 60 to measure the organic matter content from the The Scaffold Library MedChemExpress reproductive phenology of Z. noltei sediment (450 , 4.five h). by analysing sediment (OM) by loss on ignition in 1 g ofin Ria de Aveiro was assessedThen, sediment wa the spathes below a dissecting microscope and assigning them a stage of improvement separatedon their morphological traits. In sieve shaker unique SIEVING TECHNOL into seven sizes using a mechanical this way, 5 (CISA# stages of sexual primarily based OGIES BA 200 N; t = defined (Table 1, Figure1.two mm), and subsequently classified following reproduction have been 20 min, amplitude = two). Stages I, II and III corresponded towards the the period of flowering formation, gravel (2 IV andvery coarse with the period of seed sand Wentworth scale [28]: fine although stages mm), V coincided sand (1 mm), coarse formation and maturation. Broken and necrotic spathes were quantified as abortions. (0.five mm), medium sand (0.25.five mm), fine sand (0.125.25 mm), pretty fine sand (0.063The total number of spathes collected 0.125 mm) and silt and clay (0.063 mm). in every reproductive stage in the course of the study period was calculated to describe the basic reproductive phenology of Z. noltei within the Ria de Aveiro lagoon. To test differences among meadows in the reproductive phenology, the 2.two. Flowering Work and Reproductive Phenology percentage of spathes in each and every reproductive stage was estimated by meadow and date.To study the flowering effort and the reproductive phenology of Z. noltei in the Ria de Aveiro lagoon, seagrass corers (n = four; 9 cm of diameter, 6.five cm of length) have been randomly collected at 5 dates (June, July, August, September and November) in every meadow a the flowering period persisted. In the laboratory, seagrass corers had been cleaned from fauna and sediment with artificial seawater (30 psu). Then, the sexual spathes contained in eachPlants 2021, ten, x FOR PEER REVIEW15 ofPlants 2021, 10,Table 1. Description of the 5 sexual reproductive stages employed to study the reproductive phenology of Z. noltei in Ria de Aveiro. The morphology of every single reproductive stage is shown in Figure two.4 ofTable 1. Description of the 5 sexual reproductive stages utilized to study the reproductive phenology of Z. noltei in Ria de Period Stage Description Aveiro. The morphology of each reproductive stage is shown in Figure two. Yellow-green spathe, sheath closed; pistils and stamina are IEM-1460 Data Sheet visible, aligned onto I the stem Period Stage Description Pistils (IIa) and/or stamina erected (IIb); styles and stigma and/or anthers are Flowering II Yellow-green spathe, sheath closed; pistils and stamina are visible, aligned onto the stem I outside the sheath Flowering II Pistils (IIa) and/or stamina erected (IIb); styles and stigma and/or anthers are outside the sheath Stigma brown, get started to depart from spathe; typically with stamina already detached III III Stigma brown, start off to depart from spathe; generally with stamina already detached in the spathe from the spathe IV Green spathe with immature seeds; sheath closed IV Green spathe with immature seeds; sheath closed Seed formation Green/brown spathe, dark brown.
