Rlier [20,21,23].H-MRS for the quantification of intrahepatic lipid content (IHLC). The hepatocellular lipids were measuredtime of 2 sec were used. Flexible body array coil provided by system manufacturer were used for signal reception. The intrahepatic lipid content were calculated as a ratio of the sum of intensities of -(CH2)n- (1.25 ppm) and CH3- (0.8?.9 ppm) group resonances to the intensity whole MRS signal including the water resonance. Intensities of lipid and water get FCCP resonance lines were corrected for the spin-lattice (T1) and spin-spin (T2) relaxation already published T1 and T2 relaxation times at 3T [24].Analysis of Plasma MetabolitesAll laboratory parameters were measured by routine lab methods (http://www.kimcl.at/).Statistical AnalysisAll data are presented as mean 6 standard error of the mean (SEM). The effect of the insulin treatment on heart function and metabolic parameters was determined by the two-sided, paired Student’s t test. Differences at p,0.05 were considered significant. Pearson and Spearman analyses were performed to disclose correlation between variables as appropriate (SPSS 18.0 for Mac, Chicago, IL, USA).Results Clinical ParametersThe mean age was 5662 years and the mean MYCL content was 0.5860.09 of water signal. Interestingly, when IT was compared to OT, significantly lower MYCL content (0.4160.12 vs. 0.8060.11 of water signal; p = 0.034) and longer diabetes diagnosis duration (962 vs. 361 years; p = 0.015) was revealed inapplying similar short echo time 1H-MRS single voxel technique using volume of interest of 36363 cm3 placed in the lateral aspect of the liver. Single Cyproconazole manufacturer breath 1480666 hold protocol without spectral water suppression and twelve acquisitions (NS = 4) with the repetitionInsulin Alters Myocardial Lipids and Morphologythe IT-group. There were no significant differences in blood glucose levels, HbA1c, and lipid profiles at the baseline between the IT- and the OT-group (Table 1). Mean blood glucose concentrations 1676428 significantly decreased during IT (Table 1; Figure 1a). Although lipid-lowering therapy was not modified, serum cholesterol concentrations diminished after short term of IT and did not rebound at follow up (Table 1). Figure 1 presents the time course of daily insulin doses (b) as well as systolic and diastolic blood pressure (c) during the inpatient treatment. At follow up (181649 days after the initiation of IT) a reduction in HbA1c (8.360.4 ; p = 0.004) documented improved metabolic control (Table 2). Moreover, a positive correlation was found between albumin-creatinine-quotient and the following clinical features: duration of the disease (Pearsons r = 0.59; p = 0.012), plasma glucose (Pearsons r = 0.74; p = 0.001) and HbA1c levels (Pearsons r = 0.51; p = 0.036) as well as MYCL concentration at day 1 (Pearsons r = 0.52; p = 0.029).Cardiac Function and MorphologyTen days after the initiation of IT alterations in myocardial mass (+13 ) and wall thickness at the end-diastole (+13 ) were observed (Table 2). Moreover, cardiac remodeling, displayed by concentricity, emerged after the initiation of IT (Table 2). However, left ventricular systolic function did not change during the study course (Table 2). In 12 patients E/A ratio was below 1 indicating diastolic dysfunction, which remained stable under IT. The rise in myocardial mass persisted throughout the follow up period (Table 2).Cardiac and Hepatic Lipid Content during and after ITAfter 10 days of IT MYCL content increased by 80 (p = 0.008;.Rlier [20,21,23].H-MRS for the quantification of intrahepatic lipid content (IHLC). The hepatocellular lipids were measuredtime of 2 sec were used. Flexible body array coil provided by system manufacturer were used for signal reception. The intrahepatic lipid content were calculated as a ratio of the sum of intensities of -(CH2)n- (1.25 ppm) and CH3- (0.8?.9 ppm) group resonances to the intensity whole MRS signal including the water resonance. Intensities of lipid and water resonance lines were corrected for the spin-lattice (T1) and spin-spin (T2) relaxation already published T1 and T2 relaxation times at 3T [24].Analysis of Plasma MetabolitesAll laboratory parameters were measured by routine lab methods (http://www.kimcl.at/).Statistical AnalysisAll data are presented as mean 6 standard error of the mean (SEM). The effect of the insulin treatment on heart function and metabolic parameters was determined by the two-sided, paired Student’s t test. Differences at p,0.05 were considered significant. Pearson and Spearman analyses were performed to disclose correlation between variables as appropriate (SPSS 18.0 for Mac, Chicago, IL, USA).Results Clinical ParametersThe mean age was 5662 years and the mean MYCL content was 0.5860.09 of water signal. Interestingly, when IT was compared to OT, significantly lower MYCL content (0.4160.12 vs. 0.8060.11 of water signal; p = 0.034) and longer diabetes diagnosis duration (962 vs. 361 years; p = 0.015) was revealed inapplying similar short echo time 1H-MRS single voxel technique using volume of interest of 36363 cm3 placed in the lateral aspect of the liver. Single breath 1480666 hold protocol without spectral water suppression and twelve acquisitions (NS = 4) with the repetitionInsulin Alters Myocardial Lipids and Morphologythe IT-group. There were no significant differences in blood glucose levels, HbA1c, and lipid profiles at the baseline between the IT- and the OT-group (Table 1). Mean blood glucose concentrations 1676428 significantly decreased during IT (Table 1; Figure 1a). Although lipid-lowering therapy was not modified, serum cholesterol concentrations diminished after short term of IT and did not rebound at follow up (Table 1). Figure 1 presents the time course of daily insulin doses (b) as well as systolic and diastolic blood pressure (c) during the inpatient treatment. At follow up (181649 days after the initiation of IT) a reduction in HbA1c (8.360.4 ; p = 0.004) documented improved metabolic control (Table 2). Moreover, a positive correlation was found between albumin-creatinine-quotient and the following clinical features: duration of the disease (Pearsons r = 0.59; p = 0.012), plasma glucose (Pearsons r = 0.74; p = 0.001) and HbA1c levels (Pearsons r = 0.51; p = 0.036) as well as MYCL concentration at day 1 (Pearsons r = 0.52; p = 0.029).Cardiac Function and MorphologyTen days after the initiation of IT alterations in myocardial mass (+13 ) and wall thickness at the end-diastole (+13 ) were observed (Table 2). Moreover, cardiac remodeling, displayed by concentricity, emerged after the initiation of IT (Table 2). However, left ventricular systolic function did not change during the study course (Table 2). In 12 patients E/A ratio was below 1 indicating diastolic dysfunction, which remained stable under IT. The rise in myocardial mass persisted throughout the follow up period (Table 2).Cardiac and Hepatic Lipid Content during and after ITAfter 10 days of IT MYCL content increased by 80 (p = 0.008;.
