Product Name :
Anti-Rabbit IgG(H+L), Goat antibody(iFluor594)
Applications:
ELISA,WB,ICC/IF,Flow Cyt
Reactivity :
Rabbit IgG(H+L)
Conjugate:
iFluor594
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Rabbit IgG(H+L), Goat antibody(iFluor594) is designed for detecting rabbit IgG(H+L) specifically. Anti-Rabbit IgG(H+L), Goat antibody(iFluor594) is based on recombinant, goat IgG Fc fused single domain antibody to rabbit IgG(H+L) coupled to iFluor594. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG(H+L), Goat antibody(iFluor594) reacts with rabbit IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG. | Immunogen: Recombinant Rabbit IgG | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation) | Conjugate: iFluor594(Ex: 590nm, Em: 617nm), 3 moles iFluor594 per mole IgG | Specificity: Rabbit IgG(H+L) | Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS(pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C(Avoid freeze / thaw cycles), Protect from light | Background:Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site.{{103060-53-3} MedChemExpress|{103060-53-3} Purity & Documentation|{103060-53-3} Description|{103060-53-3} supplier} The IgG contains two heavy and light chains.{{371919-80-1} MedChemExpress|{371919-80-1} Purity & Documentation|{371919-80-1} In Vivo|{371919-80-1} custom synthesis} The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-Rabbit IgG(H+L), Goat antibody(iFluor594) is designed for detecting rabbit IgG(H+L) specifically. Anti-Rabbit IgG(H+L), Goat antibody(iFluor594) is based on recombinant, goat IgG Fc fused single domain antibody to rabbit IgG(H+L) coupled to iFluor594.PMID:29763040 Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG(H+L), Goat antibody(iFluor594) reacts with rabbit IgG(H+L) selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.
Immunogen:
Recombinant Rabbit IgG
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), Protect from light
Function:
Flow Cyt: 1:200-1:2000ICC/IF： 1:200-1:2000ELISA: 1:10000 -1:50000WB: 1:10000 -1:50000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
