(A) and CD3+ CD8+ (B) cells have been analyzed for IFN expression in mock-infected bone marrow (top row), E. muris-infected bone marrow (middle row) and infected spleens (bottom row). Representative flow plots are shown for C57BL/6 mice (left column) and MyD88-deficient mice (appropriate column) as well as the numbers above the gated area represent the average frequency and regular deviation of IFN + cells amongst CD4 T cells. Total numbers (C) and numbers of IFN+ CD4 and CD8 T (D) cells in the bone marrow are shown. Total numbers (E) and numbers of IFN+ CD4 and CD8 T (F) cells within the spleen are shown. Average numbers and the regular deviation are shown; gray bars represent mockinfected mice and open bars represent mice infected with E. muris. (G) Expression of CD44 and CD62L on CD3+ CD4+ IFN+ T cells inside the bone marrow (upper row) and spleen (bottom row) within the wild form mock and E. muris-infected wild kind and MyD88-deficient mice are shown. Numbers represent the frequencies within every single gated region. Statistically important variations are shown; # indicates variations amongst strains of mice, whereas * represent differences among mock and infected groups. No less than five mice have been assayed for each and every group in two independent experiments.NIH-PA Author ManuscriptJ Immunol. Author manuscript; available in PMC 2014 May well 01.Zhang et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 6. CD4 T cell-derived IFN is necessary for LSK expansion throughout bacterial infectionRadiation-induced mixed bone marrow chimeric mice were generated in which CD4 T cells have been unable to secrete IFN (CD4Ifng-/-); control chimeric mice contained wildtype CD4 T cells (CD4Ifng+/+).Daclatasvir (A) Representative flow cytometric plots of lineage-negative cells that had been analyzed for expression of c-Kit+, Sca-1+ in mock- and E. muris-infected chimeric mice on day 11 post-infection. The frequency of c-Kit+ Sca-1+ cells among total Linnegative cells is shown above each gate, as well as the average frequencies are shown in panel B. (C) Numbers of LSK cells in one particular leg is shown for mock- (filled bars) and E.muris-infected (open bars) chimeric mice. Statistically important differences are shown; # indicates differences among strains of mice, whereas * represent differences among mock and infected groups. This data represents a single experiment with among three and 7 mice in every single experimental group.Nelarabine J Immunol.PMID:32472497 Author manuscript; readily available in PMC 2014 May perhaps 01.Zhang et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Immunol. Author manuscript; out there in PMC 2014 May perhaps 01.Figure 7. Intrinsic MyD88 signaling is needed for CD4 T cell production of IFN productionICCS was performed on bone marrow and spleen cells from mixed chimeric mice (wild type: MyD88-deficient) on day 11 post-infection. (A) Representative flow plots of CD3+ CD4+ CD45.1- CD45.2+ cells. IFN and GFP expression are shown, plus the numbers on the plots represent the frequency of cells in each and every quadrant. (B) Cells were gated as in a and examined for BrdU. (C) Percentage of IFN+ cells among CD4 T cells is shown for wildtype (filled bars) and MyD88-/- cells (open bars) in bone marrow and spleen of mock and E.muris infected chimeric mice. (D) Percentage of BrdU+ cells among wild variety (filled bar) and MyD88-deficienct (open bar) CD4 T cells in bone marrow and spleen are shown. Statistically substantial variations are shown; # indicates variations among strains of mice, whereas * repres.
