Ge), specially designed to separate urine and faeces andS. no. 1 two three four 5 groups Handle (10 ml/Kg b. wt) Normal (Frusemide ten mg/kg b.wt) Alcoholic extract of roots of C.IL-17 Inhibitor Species pareira Low (one hundred mg/kg b.wt) Alcoholic extract of roots of C.pareira Medium (200 mg/kg b.wt) Alcoholic extract of roots of C.pareira Higher (400 mg/kg b.wt)DISCUSSIONMedicinal plants and botanicals offer you a all-natural safeguard against diseases and are a substantial remedy for specific ailments. Diuretics have proved to be extremely useful inside the therapy of mild to moderate hypertension and also in enhancing the effect of other antihypertensive agents. Diuretics HDAC8 Inhibitor supplier relieve pulmonary congestion and peripheral oedema. These agents are beneficial in lowering volume more than load and relieve orthopnea and paroxysmal nocturnal dyspnoea [19] in CCF and acute left ventricular failure. They reduce plasma volume and subsequently venous return towards the heart. This decreases the cardiac perform load, oxygen demand and plasma volume as well as decreases blood stress. Thusna+ mmol/l 113.03 + 2.16 191.05+2.09 129.40+2. total urine Vol (ml/kg b.wt/5 h) 13.45?.02 22.23?.01 15.20?. K+ mmol/l 51.09 + 1.51 87.81+1.60 64.13+1. Cl- mmol/l 82.95 + 1.42 129.06+1.67 94.42 + 1.73 109.44+1.20 121.39+2.0017.41?.02 20.46?.164.99+2.00 184.53+2.77.93+2.67 85.11+1.[Table/Fig-1]: Impact of alcoholic extract of roots of Cissampelos pareira on urine volume and electrolyte concentration in hydrated rat model in albino rats Values expressed as imply ?S.E.M.,n=6, Significance at p0.05, p0.01, p0.001, Compared with control group (A single Way ANOVA followed by Dunnetts `t’ test).Journal of Clinical and Diagnostic Analysis. 2014 Could, Vol-8(five): HC01-HCjcdr.netSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratssaponins, organic acids [1,17], steroids, carbohydrates, tannins, phenolic compounds, terpenoids [22], alkaloids [23], glycosides [24], sterols [25], sesquiterpenes aminoacids, carotinoids [26] in different plant extracts. Alcoholic extract of roots of Cissampelos pareira was identified with most of these plant phytochemical substances mentioned above. Hence it might be reported that the observed diuretic activity is resulting from these above phytoconstituents.CONCLUSIONResults showed that single dose administration of alcoholic extract of roots of Cissampelos pareira as one hundred,200 and 400 mg/Kg and normal Furosemide (ten mg/kg) have enhanced the urinary output along with an increase in concentration of Sodium, Potassium and Chloride ions in urine. Alcoholic extract of roots of Cissampelos pareira mg/Kg produced a greater diuretic activity that is comparable to that of typical Furosemide (ten mg/kg). In conventional medicine the plant is used for its diuretic activity. Ours scientific study come up with identification of so many phytoconstituents reported earlier for this diuretic effect in our alcoholic extract of roots of Cissampelos pareira. Thus our study supports and justifies the rationale behind the folklore use of roots of C.pareira for its diuretic activity.[Table/Fig-2]: Effect of alcoholic extract of roots of c. pareira on urinary sodium, pot assium, chloride (mmol/l) ions concentration in hydrated rat model in albino ratsACkNOwLEDGEMENTSThe authors are very considerably grateful to Dr. S.M.Shanth Kumar Ex Principal, V.L. College of Pharmacy and Dr. V.Hemanth Kumar Principal V.L. College of Pharmacy, Raichur, Karnataka for giving the laboratory facil.
