ntributions NM, GJD, HSO, and JGB developed and planned the research. MH ready fungal cultures. CB and SS prepared activitybased GlyT1 Purity & Documentation probes applied in this study. NM collected secretome samples and performed activitybased protein profil ing experiments. NM collected and analysed proteomic data. DN performed bioinformatic analysis. NM and MS ready P. pastoris strains, created and purified recombinant enzymes, and performed activity assays. NM wrote the manuscript with input from all the authors. All authors read and authorized the final manuscript. Funding The authors thank the Natural Sciences and Engineering Study Council of Canada (PostDoctoral Fellowship to NGSM), the Royal Society (Ken Murray Research Professorship to GJD), the Biotechnology and Biological Sciences Investigation Council (BBSRC) (grant BB/R001162/1 to GJD), the French National Study Agency (ANR13BIME0002 to JGB), the CK1 Molecular Weight Netherlands Organization for Scientific Analysis (NWO Major grant 2018714.018.002 to HSO), and also the European Investigation Council (ERC2011AdG290836 “Chembiosphing” to HSO, ERC2020SyG951231 “Carbocentre” to GJD and HSO). Proteomics data have been collected at the York Centre of Excellence in Mass Spectrometry, which was developed thanks to a major capital investment through Science City York, sup ported by Yorkshire Forward with funds from the Northern Way Initiative, and subsequent assistance from EPSRC (EP/K039660/1; EP/M028127/1). Availability of information and materials Pichia pastoris strains and samples of recombinant proteins may well be out there from Gideon Davies ([email protected]). Samples of ABPCel, ABPXyl, and ABPGlc may well be available from Herman Overkleeft (h.s.overkleeft@lic. leidenuniv.nl). Basidiomycete fungi are available from the fungal culture collection with the International Centre of Microbial Sources (CIRMCF) at the French National Institute for Agricultural research (INRA; Marseille, France). Genome sequences for each on the fungi made use of in this study are readily available from Mycocosm (mycocosm.jgi.doe.gov/mycocosm/home) (DOE Joint Genome Institute, Walnut Creek, California). Other datasets used and/or ana lysed through the existing study are obtainable in the corresponding author on affordable request.Author specifics 1 York Structural Biology Laboratory, Division of Chemistry, The University of York, Heslington YO10 5DD, York, UK. 2 Leiden Institute of Chemistry, Leiden University, Einsteinweg 55, 2300 RA Leiden, The Netherlands. 3 UMR1163 Bio diversitet Biotechnologie Fongiques, Facultdes Sciences de Luminy, INRAE, Aix Marseille Univ, 13288 Marseille, France. 4 Polytech Marseille, Aix Marseille Univ, 13288 Marseille, France. Received: eight October 2021 Accepted: 6 JanuaryDeclarationsEthics approval and consent to participate Not applicable. Consent for publication Not applicable. Competing interests The authors declare no competing interests.References 1. Scheller HV, Ulvskov P. Hemicelluloses. Annu Rev Plant Biol. 2010;2(61):2639. 2. Luis AS, Briggs J, Zhang X, Farnell B, Ndeh D, Labourel A, et al. Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. Nat Microbiol. 2018;three(two):210. 3. Celiska E, Nicaud JM, Bialas W. Hydrolytic secretome engineering in Yarrowia lipolytica for consolidated bioprocessing on polysaccharide sources: evaluation on starch, cellulose, xylan, and inulin. Appl Microbiol Biotechnol. 2021;105(3):9759. four. Schlembach I, Hosseinpour Tehrani H, Blank LM, B hs J, Wierckx N, Regestein L, et al. Consolidate
