Ained aggregation-competent tau. Summary/Conclusion: Exosomes contain aggregating-competent tau, but further research will be required to examine the possible for taucontaining exosomes to seed aggregation in recipient cells. Funding: This function was supported in part by the Intramural Study System with the National Institute on Aging, NIH (Dimitrios Kapogiannis, Maja Mustapic), and by grants to Dominic M. Walsh from the Alzheimer’s Drug Discovery Foundation as well as the Harvard NeuroDiscovery Center by means of a major present from Rick and Nancy Moskovitz.LBF06.Development of MSC-NTF cell exosomes for the remedy of neurodegenerative diseases Haggai Kaspi; Jonathan Semo; Natalie Abramov; Revital Aricha Brainstorm Cell Theraputics, Petach Tikva, IsraelBackground: MSC-NTF cells are autologous bone marrow derived mesenchymal stem cells (MSC) induced below culture conditions to create high levels of neurotrophic variables and miRNAs that support NOD-like Receptor Proteins medchemexpress neuronal development and survival, leveraging the potential therapeutic rewards of MSCs that involves anti-inflammatory and immunomodulatory properties. MSC-NTF derived exosomes may possibly possess a exceptional features for the enhanced cell to cell delivery of therapeutics towards the brain, which includes proteins, microRNA, RNA along with other signalling molecules, resulting from their ability to cross the blood rain barrier and distribute widely inside the brain and spinal cord. Procedures: We developed a high scale course of action to isolate high-purity solution of exosomes secreted by MSC-NTF cells working with tangential flow filtration. Quantification, size and Tissue Inhibitor of Metalloproteinase (TIMPs) Proteins Purity & Documentation integrity had been verified by zetaview and transmission electron microscopy. Immunomodulatory and neuroregenerative properties were studied in vitro on peripheral blood mononuclear cells and neural precursor cells. Outcomes: MSC-NTF derived exosomes inhibited T cells proliferation, decreased secretion of inflammatory cytokines and elevated the proportion of Treg cells inside a dose-dependent manner. Culture of neural precursor cells with exosomes accelerated neuronal differentiation and neurite outgrowth in a dose-dependent manner. Summary/Conclusion: MSC-NTF cells-derived exosomes might leverage the advantages with the cells of origin by providing an option to deliver bioactive molecules to the brain inside a non-invasive administration, thus generating a novel and promising therapeutic technique for neurodegenerative ailments.Saturday, 05 MayScientific System ISEV2018 Saturday, 05 Could 2018 Symposium Session 19 Cross-organism Communication in Bacterial Infections Chairs: Igor Almeida; Cherie Blenkiron Location: Auditorium 08:300:OS19.Transcytosis of extracellular vesicles made by Bacillus subtilis 168 in human intestinal Caco-2 cell monolayers Ana Paula Dom guez Rubio; Jimena Hebe. Mart ez; Marcos Palavecino; Mariana Piuri; Oscar Edgardo P ez Departamento de Qu ica Biol ica, Universidad de Buenos Aires, Ciudad Aut oma de Buenos Aires, ArgentinaBackground: Bacillus subtilis 168 is actually a normal resident in the mammalian gastrointestinal (GI) microbiome and has been applied in food fermentations, becoming awarded the status of “Generally Recognized As Safe” (GRAS). Extracellular vesicles (EVs) have been proposed to be involved in signalling amongst probiotic bacteria and their mammalian hosts. B. subtilis 168 produces EVs which have been on the nanometric size variety (5000 nm). EVs carried cytoplasmic elements, for example specific proteins, which suggest a function for the EVs within the bacteria I cells interface. We hypothesize that transc.
