Fter TLC-DB and the extraction parameters of SCF process. P. aeruginosa Sample 1 2 three 4 five six 7 eight 9 10 11 12 13 14 15 16 17 18 19 Diameter (mm) 5.86 5.50 7.51 six.18 five.43 0.00 4.29 4.83 0.00 6.50 six.91 six.27 5.41 five.71 0.00 6.37 six.56 0.00 five.MRSA SD Diameter (mm) 5.46 four.85 7.57 four.69 four.80 0.00 four.66 4.32 0.00 4.59 4.77 three.29 3.41 4.25 0.00 3.50 three.29 0.00 four.SDMPa 20 15 10 20 15 ten 20 15 10 20 15 10 20 15 ten 20 15 10CEtOH 2 2 two 2 two two two 2 two 1.5 1.5 1.five 1.5 1.5 1.five 1.5 1.five 1.50.72 0.56 0.85 0.64 0.42 0.00 0.71 0.29 0.00 0.42 0.20 0.33 0.47 0.80 0.00 0.78 0.55 0.00 0.0.50 0.78 0.62 0.46 0.38 0.00 0.39 0.59 0.00 0.65 0.69 0.42 0.36 0.35 0.00 0.40 0.46 0.00 0.40 40 40 60 60 60 80 80 80 40 40 40 60 60 60 80 80 80Molecules 2021, 26,4 ofTable 1. Cont. P. aeruginosa Sample 20 21 22 23 24 25 26 27 chloroform ch EtOH EtOH Vancomycin Gentamicin Diameter (mm) 6.34 five.32 5.61 four.98 0.00 3.02 two.75 0.00 0.00 0.00 0.00 eight.MRSA SD Diameter (mm) 3.58 three.72 3.72 three.07 0.00 three.40 3.25 0.00 0.00 0.00 0.00 12.30SDMPa 15 10 20 15 ten 20 15CEtOH 1 1 1 1 1 1 10.82 0.41 0.47 0.61 0.00 0.23 0.48 0.00 0.00 0.00 0.00 0.0.82 0.40 0.40 0.36 0.00 0.58 0.68 0.00 0.00 0.00 0.00 0.45 -40 40 60 60 60 80 80SD = typical deviation; ch = chloroform.Figure 1. (a) Detection of the antibacterial activity of clary sage SC-CO2 extracts, optimistic manage (gentamicin), and solvents against P. aeruginosa by TLC-DB. The Olesoxime site numbering of your samples is the very same as that shown in Table 1; (b) Detection of the antibacterial activity of clary sage SC-CO2 extracts, positive control (vancomycin), and solvents against MRSA by TLC-DB. The numbering of the samples could be the similar as that shown in Table 1.Molecules 2021, 26,five ofFigure two. Antibacterial activity of every single clary sage extracts and gentamicin as good manage on P. aeruginosa. The numbering with the samples will be the identical as that shown in Table 1.Figure 3. Antibacterial activity on MRSA of every single clary sage extracts and vancomycin as constructive handle. The numbering with the samples will be the identical as that shown in Table 1.Molecules 2021, 26,6 of2.2. Response Surface Final results The response surface models with the extracts were -Irofulven In Vitro plotted by utilizing the Design and style Expert program. Figures four and five show how the antibacterial activity in the extract modifications against bacterial strains of P. aeruginosa and MRSA as well as the constant alter of specific parameters. It can be observed that within the case of P. aeruginosa, the diameter with the zones of inhibition was larger on typical, so the extracts have been additional effective against this sort of bacterium. The shape from the response surfaces is quite similar in the two cases studied. As the figures show, the ideal results were obtained at low temperatures (405 C) and moderately higher pressures (159 MPa). The least successful extracts is usually ready at 400 C and 101 MPa. Throughout the extraction, the plant material should be protected from excessive heat. As a function of your cosolvent ratio, the shape of the figures is much less characteristic, from which it may be concluded that in the three setting parameters, the EtOH concentration has the least impact around the antibacterial activity. Inside the case of P. aeruginosa, the optimal ratio may be amongst 1.six and 1.7 , in addition to a further raise inside the EtOH ratio will no longer produce a important improvement in antibacterial activity. Having said that, inside the case of MRSA, the very best benefits could be obtained having a cosolvent ratio of 2 . For each bacterial strains, the worst results were obtained at a ratio of 1.0.two EtOH.Figure 4.
