Fter TLC-DB and also the extraction parameters of SCF procedure. P. aeruginosa Sample 1 two three four five six 7 eight 9 ten 11 12 13 14 15 16 17 18 19 Diameter (mm) five.86 5.50 7.51 six.18 five.43 0.00 four.29 four.83 0.00 six.50 six.91 6.27 five.41 5.71 0.00 6.37 six.56 0.00 five.MRSA SD Diameter (mm) 5.46 four.85 7.57 four.69 4.80 0.00 4.66 four.32 0.00 four.59 four.77 3.29 three.41 4.25 0.00 3.50 three.29 0.00 four.SDMPa 20 15 ten 20 15 ten 20 15 ten 20 15 10 20 15 ten 20 15 10CEtOH two two 2 2 two two 2 2 2 1.5 1.five 1.5 1.5 1.5 1.5 1.five 1.five 1.50.72 0.56 0.85 0.64 0.42 0.00 0.71 0.29 0.00 0.42 0.20 0.33 0.47 0.80 0.00 0.78 0.55 0.00 0.0.50 0.78 0.62 0.46 0.38 0.00 0.39 0.59 0.00 0.65 0.69 0.42 0.36 0.35 0.00 0.40 0.46 0.00 0.40 40 40 60 60 60 80 80 80 40 40 40 60 60 60 80 80 80Molecules 2021, 26,4 ofTable 1. Cont. P. aeruginosa Sample 20 21 22 23 24 25 26 27 chloroform ch EtOH EtOH Vancomycin Gentamicin Diameter (mm) 6.34 five.32 5.61 4.98 0.00 three.02 2.75 0.00 0.00 0.00 0.00 8.MRSA SD Diameter (mm) 3.58 3.72 3.72 3.07 0.00 three.40 three.25 0.00 0.00 0.00 0.00 12.30SDMPa 15 10 20 15 ten 20 15CEtOH 1 1 1 1 1 1 ten.82 0.41 0.47 0.61 0.00 0.23 0.48 0.00 0.00 0.00 0.00 0.0.82 0.40 0.40 0.36 0.00 0.58 0.68 0.00 0.00 0.00 0.00 0.45 -40 40 60 60 60 80 80SD = typical deviation; ch = chloroform.Figure 1. (a) Detection of the antibacterial activity of clary sage SC-CO2 extracts, positive control (gentamicin), and solvents against P. aeruginosa by TLC-DB. The numbering of the samples would be the similar as that shown in Table 1; (b) Detection with the antibacterial activity of clary sage SC-CO2 extracts, good control (vancomycin), and solvents against MRSA by TLC-DB. The numbering of the samples would be the same as that shown in Table 1.Molecules 2021, 26,5 MRTX-1719 Technical Information ofFigure 2. Antibacterial activity of every clary sage DMPO MedChemExpress extracts and gentamicin as positive control on P. aeruginosa. The numbering with the samples could be the exact same as that shown in Table 1.Figure 3. Antibacterial activity on MRSA of each and every clary sage extracts and vancomycin as positive manage. The numbering from the samples could be the same as that shown in Table 1.Molecules 2021, 26,six of2.2. Response Surface Outcomes The response surface models of the extracts had been plotted by using the Style Expert plan. Figures four and five show how the antibacterial activity with the extract changes against bacterial strains of P. aeruginosa and MRSA along with the continuous modify of specific parameters. It can be seen that within the case of P. aeruginosa, the diameter with the zones of inhibition was larger on average, so the extracts have been extra productive against this kind of bacterium. The shape from the response surfaces is very similar within the two cases studied. As the figures show, the very best final results were obtained at low temperatures (405 C) and moderately higher pressures (159 MPa). The least successful extracts may be prepared at 400 C and 101 MPa. During the extraction, the plant material has to be protected from excessive heat. As a function from the cosolvent ratio, the shape with the figures is significantly less characteristic, from which it can be concluded that from the 3 setting parameters, the EtOH concentration has the least effect on the antibacterial activity. Within the case of P. aeruginosa, the optimal ratio may well be in between 1.6 and 1.7 , and a further enhance inside the EtOH ratio will no longer produce a considerable improvement in antibacterial activity. Having said that, within the case of MRSA, the best outcomes might be obtained having a cosolvent ratio of 2 . For both bacterial strains, the worst final results had been obtained at a ratio of 1.0.two EtOH.Figure four.
