Nd [54]. Furthermore, 5-azaC may also inhibit RNA methylation, E.K., R.T. and T.A.R.; CC-90011 Cancer validation, regulatory layer for chondrogenic differentiation [55]. Consequently, it is affordable to another R.Z. and T.A.R.; formal evaluation, J.V., E.K., L.D. and T.A.R.; investigation, J.V., K.K., K.K. and T.A.R.; sources, R.Z., T.A.R. and C.M.; writing–original draft preparation, J.V., E.K., C.M., R.Z., think about that alternative when the effect of 5-azaC treatment is evaluated.Cells 2021, ten,18 ofSupplementary Materials: The following are offered on the internet at https://www.mdpi.com/article/10 .3390/cells10102678/s1, Table S1: Sequences of primer pairs applied for the PCR array analyses, Table S2: Sequences of primer pairs applied for the RT-qPCR reactions, Table S3: Sequences of primer pairs used for the qMSP analyses, Table S4: Sequence information of the three UTR regions of Dnmt3a, Ogt and Tet1 genes with insert flanking T7 promoters for antisense probe preparation, Figure S1: Photomicrograph of an E15 mouse embryo applied for in situ hybridization as a negative manage (no distinct RNA probe was used), Figure S2: Quantitative (relative optical density) values with the Dnmt3a-, Tet1- and Ogt-specific in situ hybridization photomicrographs. Author Contributions: Conceptualization, R.Z. and T.A.R.; methodology, J.V., E.K., R.T. and T.A.R.; validation, R.Z. and T.A.R.; formal analysis, J.V., E.K., L.D. and T.A.R.; investigation, J.V., E.K., K.K. and T.A.R.; resources, R.Z., T.A.R. and C.M.; writing–original draft preparation, J.V., E.K., C.M., R.Z., T.A.R.; writing–review and editing, J.V., R.Z., T.A.R., C.M.; visualization, J.V., E.K. and T.A.R.; supervision, R.Z., T.A.R.; project administration, R.Z.; funding acquisition, R.Z., C.M., T.A.R. All authors have read and agreed for the published version of your manuscript. Funding: This perform was supported by the 2020/R/20/2502 Gedeon Richter Plc. grant awarded to J.V. The publication was supported by the EFOP-3.six.1-16-2016-00022 and the EFOP-3.6.3-VEKOP-16-201700009 projects, the projects are co-financed by the European Union and the European Social Fund. C.M. was supported by the Premium Postdoctoral Analysis Fellowship with the E v Lor d Investigation Network (ELKH), plus the Young Researcher Excellence Programme (grant quantity: FK-134304) in the National Study, Improvement and Innovation Office, Hungary. Project no. TKP2020-NKA04 has been implemented using the assistance offered in the National Investigation, Improvement and Innovation Fund of Hungary, financed under the 2020-4.1.1-TKP2020 funding scheme. T.A.R. received funding from the National Study, Development and Innovation Fund, Hungary (grant quantity: K131588). Institutional Overview Board Statement: The study was conducted in line with the suggestions of the Declaration of Helsinki, and authorized by the Animal Care and Protection Committee in the University of Debrecen (2/2018/DEM ). Informed Consent Statement: Not applicable. Information Availability Statement: The data presented in this study are out there on request from the corresponding author. Acknowledgments: The authors would like to thank Krisztina B in the Department of Anatomy, Histology and Embryology for their skillful and great technical assistance. Conflicts of Interest: The authors Xanthoangelol custom synthesis declare that they’ve no competing interests. This paper was written by the authors within the scope of their academic and analysis positions. None of your authors have any relationships that may be construed as biased or inappropriate. The f.
