Ultural University, 611130, Chengdu, China; 3 Institute of Chinese Healthcare Sciences, State Crucial Laboratory of High-quality Study in Chinese Medicine, University of Macau, Avenida da Universidade, Taipa, Macau Correspondence: Shun Gao [email protected] Journal of Chinese Medicine 2018, 13(Suppl 1):127 Background: The utilization of wastes of agricultural and industrial residues as a source of functional components is of good interest. Lepidium meyenii (2-Aminoethyl)phosphonic acid Biological Activity leaves are a byproduct of L. meyenii and include goodquality extractable polyphenols. Materials and approaches: The effects of ethanol concentration, mixing time, solvent/meal ratio and temperature have been studied on the extraction of polyphenols from L. meyenii leaves. In addition, the isolation measures and antioxidant activity of L. meyenii leaves polyphenols had been also made. Results: Results suggested that polyphenols yield was significantly impacted by ethanol concentration, mixing time, and solvent/meal ratio. Determined by the screening benefits of extraction parameters, a central composite style was applied with 3 variables: ethanol concentration (20, 40, and 60 ), extraction time (30, 90 and 150 min); and solvent/meal ratio (10:1, 25:1 and 40:1). Selected response which evaluates the extraction process was polyphenol yield and the second-order model obtained for polyphenol yield revealed coefficient of determination of 98.8 . Polyphenol yield obtained ranged from 27.83 to 30.65 mg/g. Maximum yield (30.86 mg/g) was obtained when ethanol concentration, extraction time and solvent/meal ratio have been 47.78 , 76.99 min and 1: 21.six, respectively. Experiments at optimum circumstances gave a polyphenol yield of 30.36 mg/g, suggesting that the relative error of predictive worth is only 1.62 . According to the optimum extraction parameters, the isolation and purification actions consisted of extracting polyphenol in 47.78 ethanol, followed by centrifugation, plate filtration, ultrafiltration, and vacuum concentration. two.58 g of polyphenols may be obtained from 100 g leaves, as well as the recovery price ��-Cyano-4-hydroxycinnamic acid Monocarboxylate Transporter reached 85.1 . Results from antioxidant activity tests showed that the polyphenol may perhaps proficiently scavenge DPPH totally free -?radical, H and O2 , along with the dose ffect relation is notable. The cost-free radical scavenging ability of L. Meyenii leaves polyphenol was larger than that of BHT. Moreover, the scavenging potential are distinctive, and -?the capacities from powerful to weak are DPPH no cost radical, O2 and H. Conclusions: These findings will help to design the method of optimal polyphenol extraction and isolation from L. meyenii leaves. 128 Determination and evolution of sesame lignans in Monascus aged vinegar employing microwave irradiation Yazhen Chen, Jicheng Chen, Jingjing Tian, Huifang Ge, Hetong Lin College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China Correspondence: Jicheng Chen [email protected]; Hetong Lin [email protected] Journal of Chinese Medicine 2018, 13(Suppl 1):128 Background: To establish a HPLC system for the simultaneous analysis of 3 sesame lignans which includes sesamol, sesamin and sesamolin in Monascus aged vinegar. Furthermore, the created process was applied for investigating the conversion from sesamolin to sesamol over microwave irradiation.Chin Med 2018, 13(Suppl 1):Web page 58 ofMaterials and strategies: The chromatography was performed on Waters XBridge C18 reversed phase column (250 mm ?four.6 mm, five m) with methanol ater (gradient elution) because the mobile phase at 40 .
