Tity of every single residue pair in each and every column, with green bars representing identical residues and missing bars highlighting places where sequences differ. End gaps within the alignments have strong green bars. Amino acids are shaded based on similarity, with black representing identical residues among the two sequences, grey representing similar residues, and white representing dissimilar residues. A = arrestin, B = cGMP gated channel alpha, C = cGMP gated channel beta, D = Goprotein alpha subunit, E = Gtprotein alpha subunit, F = Gprotein beta subunit, G = Gprotein gamma subunit, H = opsin, I = PDE alpha, J = PDE beta, K = protein kinase A, L = rhodopsin kinase. (PDF) Figure S3 Amino acid alignments of scallop circadian clock genes to recognized homologs. Circadian clock gene sequences from the Placopecten magellanicus adult eye transcriptome were translated and aligned to identified homologs from Drosophila, mouse, or Crassostrea gigas. Alignments had been completed and exported from Geneious v. 5.6 (www.geneious.com). The graph above the alignment represents imply pairwise identity of each and every residue pair in each column, with green bars representing identical residues and missing bars highlighting places exactly where the sequences differ. End gaps in the alignments have solid green bars. Amino acids are shaded D-Fructose-6-phosphate (disodium) salt Endogenous Metabolite according to similarity, with black representingidentical residues amongst the two sequences, grey representing similar residues, and white representing dissimilar residues. A = clock, B = cryptochrome, C = cycle, D = doubletime, E = period, F = timeless. (PDF)Table SList of phototransduction and circadian clock protein sequences used in blasts to identify homologs within every scallop eye transcriptome. All protein sequences have been downloaded from NCBI and blasted against the transcriptomes utilizing Geneious v. five.five (Biomatters). (XLSX)Table S2 Genes annotated with Gene Ontology (GO) terms related to neural processes, vision, or retina(l) in the adult eye transctriptome of A. irradians. (XLSX) Table S3 Genes annotated with Gene Ontology (GO) terms associated with neural processes, vision, or retina within the adult eye transcriptome of P. magellanicus. (XLSX) Table S4 List of genes identified in KEGG pathways associated with light (S)-(-)-Propranolol GPCR/G Protein detection inside the adult eye transcriptomes of A. irradians and P. magellanicus. KEGG pathways have been identified applying the KEGG Automatic Annotation Server (www. genome.jp/tools/kaas). (XLSX)AcknowledgmentsBrad Fleming kindly assisted with MATLAB scripts to organize information files and Fadi Towfic assisted using the InParanoid analyses. Figure 1 was developed by Samantha Butler. The photo for Figure 1A was taken by Anita Krause. We thank Keithanne Mockaitis for her help with production in the Argopecten irradians transcriptome dataset. We also thank members in the D.C. Adams, N. Valenzuela, and J.M. Serb labs and 3 anonymous reviewers for their valuable comments on preceding drafts of this manuscript.Author ContributionsConceived and created the experiments: ANP JMS. Performed the experiments: ANP. Analyzed the data: ANP JMS. Contributed reagents/ materials/analysis tools: JMS. Wrote the paper: ANP JMS. Completed the scallop eye tissue dissections: ANP JMS.
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