Mmended by the manufacturers (n = 10 for each treatment group). Pouch fluid
Mmended by the manufacturers (n = 10 for each treatment group). Pouch fluid was harvested by syringe at 1, 6, 12, 24 and 48 hours post LPS administration and subjected to histological and cytokine/cytokine receptor analysis. TNF and sTNFR1 levels were determined by ELISA and an immunofluorescent dot blot assay. Results Pouch fluid analysis: JNJ-26481585 supplier maximal effects were detected at 6 hours post LPS administration. TNF levels were significantly depressed in animals dosed with HFP, but not in animals treated with FFP or carprofen (P <0.05). sTNFR1 levels were significantly elevated in HFP, but not in FFP or carprofen dosed animals (P <0.05). Neutrophil numbers were significantly depressed in HFP dosed but not in FFP or carprofen treated animals (P <0.05). Conclusions There appears to be a correlation between elevated levels of sTNFR1 and depression of TNF and neutrophil levels in the pouch fluid of HFP dosed rats (r = ?.73, P <0.0001). The data suggest that canine HFP, which has been demonstrated to contain elevated levels of sTNFR1 compared with FFP, has a direct effect on depressing TNF levels and neutrophil sequestration in the rat air pouch model of inflammation. These data suggest that HFP may be worthy of further investigation to determine whether such preparations have a therapeutic potential for treatment of acute inflammatory diseases in which TNF is implicated.P9 Clinical impact of a PCR-based assay for pathogen detection in critically ill patients with evidence of infectionF Bloos1, A Kortgen1, S Sachse2, M Lehmann3, E Straube2, K Reinhart1, M Bauer1 1Department of Anesthesiology and Intensive Care Medicine, University Hospital Jena, Germany; 2University Hospital Jena, Institute of Medical Microbiology, Jena, Germany; 3SIRS-Lab GmbH, Jena, Germany Critical Care 2009, 13(Suppl 4):P9 (doi: 10.1186/cc8065) Introduction Blood cultures are often negative even in patients with clinical signs of severe sepsis. Furthermore, the long time to result of culture-based methods does not allow the results to guide empiric antimicrobial therapy. PCR-based pathogen detection promises a higher rate of positivity and a faster time to result. Objective To report the performance of PCR-based pathogen detection compared with blood culture in ICU patients with evidence of infection, and the impact of this test on the antimicrobial therapy. Methods Patients treated on an interdisciplinary ICU were included into this observational study if a blood culture (BC) wasP8 Effect of canine hyperimmune plasma on TNF and inflammatory cell levels in a lipopolysaccharide-mediated rat air pouch model of inflammationB Essien, M Kotiw, H Buttler, D Strunin Centre for Systems Biology, University of Southern Queensland, Toowoomba, Queensland, Australia Critical Care 2009, 13(Suppl 4):P8 (doi: 10.1186/cc8064)SAvailable online http://ccforum.com/supplements/13/SFigure 1 (abstract P9)P11 Abstract withdrawn P12 Abstract withdrawn P13 Comparison of commercial DNA extraction kits for the detection of bacterial genomic DNA from whole-blood samples using a broad-range PCRB Krulova, E Nemcova, B Zaloudikova, P Nemec, T Freiberger Centre for Cardiovascular Surgery and Transplantation, Molecular Genetic Laboratory, Brno, Czech Republic Critical Care 2009, 13(Suppl 4):P13 (doi: 10.1186/cc8069)Figure 2 (abstract P9)drawn on discretion of the treating physician. Blood cultures PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26740125 and EDTA-blood were taken by sterile venous puncture. The EDTAblood was processed with a PCR-based assay (VYOO? SI.
