Imilar to other toxic protein oligomers in this regard [30]. Similar, but smaller, changes in theParticles length/size (nm)Figure 2. Size distribution of Ab42CC protofibrils measured using different methods. The blue and red lines/symbols represent data from atomic force microscopy. One sample (blue) was washed briefly with deionized water, while a second sample (red) was washed extensively. The lengths of ca. 1500 protofibrils were measured in each case. The gray dashed line reflects an expected distribution corresponding to the analytical ultracentrifugation measurements (Fig. 3) assuming that Ab42CC protofibrils have a dehydrated diameter of 3.1 nm. The black line represents the distribution of apparent hydrodynamic radius obtained from nanoparticle tracking analysis using a NanoSight microscope. doi:10.1371/journal.pone.0066101.gEngineered Ab42CC Protofibrils Mimic Wild Type AbAAbsorbance at 280 nm (AU)0.8 0.6 0.4 0.Fluorescence intensity (a.u.)1.free ANS ANS + A42CC monomer ANS + A42CC protofibril6.6.6.6.7.Radius (cm)Wavelength (nm)Figure 4. Ab42CC protofibrils expose binding sites for the ANS dye. Fluorescence emission spectra of 50 mM free ANS (red) and of ANS in the presence of Ab42CC protofibrils (black) or monomeric Ab42CC (green). Peptide concentrations are in both cases 10 mM monomer units. doi:10.1371/journal.pone.0066101.gB0.c(s) distribution (1/S)0.0.00 5 10 15 20 25 30 35S20, W (S)Figure 3. Size distribution of Ab42CC protofibrils in solution monitored by analytical ultracentrifugation. (A) A subset of the raw sedimentation velocity centrifugation data of 300 mM Ab42CC protofibrils at 20uC recorded over a period of 20 h. (B) Sedimentation Title Loaded From File coefficient distribution of Ab42CC protofibrils analyzed using a continuous c(s) distribution model. doi:10.1371/journal.pone.0066101.gand Title Loaded From File certain Ab oligomers [21,33]. Glabe et al. used the OC serum to show that Ab forms two immunologically distinct types of oligomers along two aggregation pathways: “pre-fibrillar” oligomers that are recognized by A11, but not by OC, and “fibrillar” oligomers that are recognized by OC, but not by A11 [21,33]. We performed dot blot assays for OC binding to Ab42CC protofibrils and monomeric Ab42CC that had been immobilized on nitrocellulose membranes. The OC serum recognizes Ab42CC protofibrils and wild type Ab42 fibrils to the same extent in this assay, whereas no binding can be detected to monomeric Ab42CC (Fig. 5). The assay is conformation specific because SDS-treated Ab42CC protofibrils are not recognized by OC (Fig. 5). These results place Ab42CC protofibrils in the same category of aggregated species as the fibrillar oligomers formed along the A11 negative/OC positive aggregation pathway of wild type Ab.ANS emission can be observed with monomeric Ab42CC, but it is not clear if monomeric Ab42CC also binds ANS or if small amounts of Ab42CC aggregates are present also in these samples.Ab42CC protofibrils bind Apolipoprotein E in human serumThe high stability of Ab42CC protofibrils makes them potentially useful for studies of biological processes related to AD. To test this possibility we performed a pilot pull-down study to 23977191 identify protein binders in biological fluids. Using Ab42CC protofibrils immobilized on magnetic beads we were able to extract protein ligands from human serum (Fig 6). Interestingly, the strongest (or most prevalent) binder was identified as apolipoprotein E (isoform ApoE4; 67 sequence coverage and Mascot score = 348). Sever.Imilar to other toxic protein oligomers in this regard [30]. Similar, but smaller, changes in theParticles length/size (nm)Figure 2. Size distribution of Ab42CC protofibrils measured using different methods. The blue and red lines/symbols represent data from atomic force microscopy. One sample (blue) was washed briefly with deionized water, while a second sample (red) was washed extensively. The lengths of ca. 1500 protofibrils were measured in each case. The gray dashed line reflects an expected distribution corresponding to the analytical ultracentrifugation measurements (Fig. 3) assuming that Ab42CC protofibrils have a dehydrated diameter of 3.1 nm. The black line represents the distribution of apparent hydrodynamic radius obtained from nanoparticle tracking analysis using a NanoSight microscope. doi:10.1371/journal.pone.0066101.gEngineered Ab42CC Protofibrils Mimic Wild Type AbAAbsorbance at 280 nm (AU)0.8 0.6 0.4 0.Fluorescence intensity (a.u.)1.free ANS ANS + A42CC monomer ANS + A42CC protofibril6.6.6.6.7.Radius (cm)Wavelength (nm)Figure 4. Ab42CC protofibrils expose binding sites for the ANS dye. Fluorescence emission spectra of 50 mM free ANS (red) and of ANS in the presence of Ab42CC protofibrils (black) or monomeric Ab42CC (green). Peptide concentrations are in both cases 10 mM monomer units. doi:10.1371/journal.pone.0066101.gB0.c(s) distribution (1/S)0.0.00 5 10 15 20 25 30 35S20, W (S)Figure 3. Size distribution of Ab42CC protofibrils in solution monitored by analytical ultracentrifugation. (A) A subset of the raw sedimentation velocity centrifugation data of 300 mM Ab42CC protofibrils at 20uC recorded over a period of 20 h. (B) Sedimentation coefficient distribution of Ab42CC protofibrils analyzed using a continuous c(s) distribution model. doi:10.1371/journal.pone.0066101.gand certain Ab oligomers [21,33]. Glabe et al. used the OC serum to show that Ab forms two immunologically distinct types of oligomers along two aggregation pathways: “pre-fibrillar” oligomers that are recognized by A11, but not by OC, and “fibrillar” oligomers that are recognized by OC, but not by A11 [21,33]. We performed dot blot assays for OC binding to Ab42CC protofibrils and monomeric Ab42CC that had been immobilized on nitrocellulose membranes. The OC serum recognizes Ab42CC protofibrils and wild type Ab42 fibrils to the same extent in this assay, whereas no binding can be detected to monomeric Ab42CC (Fig. 5). The assay is conformation specific because SDS-treated Ab42CC protofibrils are not recognized by OC (Fig. 5). These results place Ab42CC protofibrils in the same category of aggregated species as the fibrillar oligomers formed along the A11 negative/OC positive aggregation pathway of wild type Ab.ANS emission can be observed with monomeric Ab42CC, but it is not clear if monomeric Ab42CC also binds ANS or if small amounts of Ab42CC aggregates are present also in these samples.Ab42CC protofibrils bind Apolipoprotein E in human serumThe high stability of Ab42CC protofibrils makes them potentially useful for studies of biological processes related to AD. To test this possibility we performed a pilot pull-down study to 23977191 identify protein binders in biological fluids. Using Ab42CC protofibrils immobilized on magnetic beads we were able to extract protein ligands from human serum (Fig 6). Interestingly, the strongest (or most prevalent) binder was identified as apolipoprotein E (isoform ApoE4; 67 sequence coverage and Mascot score = 348). Sever.
