Ension is approx from 150 to 10 kD. Proteins identified as differentially expressed are indicated with yellow arrows with assigned numbers from the DeCyder analysis. The numbers in the figure correspond to those presented in Table 2; (bottom) MALDI OF S mass spectrum of spot 1543 identified as ceruloplasmin according to the matched peaks. doi:10.1371/journal.pone.0051256.gas isoforms. For example, haptoglobin and transthyretin were represented by two and three spots, respectively, and in both cases, the individual spots were similarly down-regulated. Additionally,the same protein being present in several different spots could have represented biologically relevant 
modifications or proteolytic fragments (e.g., ceruloplasmin).Table 2. Differentially expressed proteins between chemosensitive and chemoresistant ovarian cancer ascites identified by MALDI-TOF/TOF MS.Spot Noa Peptide count 33 45.4/5.28 MW/PI (kDa) 21.57 gi|93163358Protein nameRatiobAccession noc1485-00-3 manufacturer Mascot ScoredProtein functionse Antioxidants, lipid transport and metabolism, acute-phase protein, tumorf Microtubules, movement of the cell, cytoskeletal protein Transthyretin variants implicate formation of amyloid fibril Lipid transport and metabolism, lecithin cholesterol acyltransferase, acute-phase protein Recombinant proapolipoprotein A-I Histidine-rich proteolytic fragment of ceruloplasmin Carrier Proteins, oxidoreductases, acute-phase protein, tumorfApolipoprotein A-IV Precursor21.69 21.56 2.16 gi|4557321 
389 17 30.8/5.56 gi|2098257 355 8 13.9/5.35 gi|15277503 430 17 40.1/5.55 2.7 21.66 2.86 22.87 gi|48145933 121 4 gi|1620909 79 6 gi|223092 151 4 18.7/5.31 11.6/5.43 16.0/5.5 gi|178775 307 18 29.0/5.45 Thyroid hormone transport, serine proteinase inhibitors, acute-phase protein, tumorf 12.8/5.33 42.3/6.23 4 39.0/6.27 Affects the amyloid pathway Isoform of haptoglobin Recycling of heme- iron, acute-phase protein, Mucoproteins, tumorf 22.17 22.27 22.6 gi|1212947 289 gi|223976 318 6 gi|55669575 442ACTB proteinChain A, TTR VariantsApolipoprotein A-I preproproteinProapolipoproteinCeruloplasmin CpFCeruloplasminTransthyretinCovalent Dimer Of Transthyretin7 Biomarkers for Chemoresistant Ovarian CancerHaptoglobin HpHaptoglobinabSpot no. assigned by DeCyder 2-D Differential Analysis Software V6.0. corresponding to the DIGE image in Figure 1. Decreased or increased ratio of protein in ascites of intrinsic chemoresistant ovarian cancer compared to chemosensitive ones; “?’ indicates down-regulation of protein in ascites. Accession no. from NCBInr database. d Mascot scores greater than 40 1516647 were considered significant. e Protein function mainly categorized according to MeSH Tree Structure. f Alterations in the serum level may indicate: occurrence, tumor progression, response to therapy or prognosis for some malignancy reported in the literature. doi:10.1371/journal.pone.0051256.tcBiomarkers for Chemoresistant Ovarian CancerTable 3. Concentrations of proteins in ascites of intrinsically chemoresistant and chemosensitive EOC.Analytes Apo-AIV (mg/mL) CP (mg/mL) HB (mg/L) TTR (mg/L)Chemosensitive 230.3641.4 157.5611.5 263.1638.0 316.2635.Chemoresistant 215.0635.7 192.2619.5 266.6644.1 337.9636.P order AZ876 value0.349 0.001 0.832 0.*Apo-AIV, apoliprotein A-IV; CP, ceruloplasmin; HB, haptoglobin; TTR, transthyretin. doi:10.1371/journal.pone.0051256.tThe change in serum concentration of ceruloplasmin, along with many other acute phase proteins identified among the differentially expressed proteins, implicated t.Ension is approx from 150 to 10 kD. Proteins identified as differentially expressed are indicated with yellow arrows with assigned numbers from the DeCyder analysis. The numbers in the figure correspond to those presented in Table 2; (bottom) MALDI OF S mass spectrum of spot 1543 identified as ceruloplasmin according to the matched peaks. doi:10.1371/journal.pone.0051256.gas isoforms. For example, haptoglobin and transthyretin were represented by two and three spots, respectively, and in both cases, the individual spots were similarly down-regulated. Additionally,the same protein being present in several different spots could have represented biologically relevant modifications or proteolytic fragments (e.g., ceruloplasmin).Table 2. Differentially expressed proteins between chemosensitive and chemoresistant ovarian cancer ascites identified by MALDI-TOF/TOF MS.Spot Noa Peptide count 33 45.4/5.28 MW/PI (kDa) 21.57 gi|93163358Protein nameRatiobAccession nocMascot ScoredProtein functionse Antioxidants, lipid transport and metabolism, acute-phase protein, tumorf Microtubules, movement of the cell, cytoskeletal protein Transthyretin variants implicate formation of amyloid fibril Lipid transport and metabolism, lecithin cholesterol acyltransferase, acute-phase protein Recombinant proapolipoprotein A-I Histidine-rich proteolytic fragment of ceruloplasmin Carrier Proteins, oxidoreductases, acute-phase protein, tumorfApolipoprotein A-IV Precursor21.69 21.56 2.16 gi|4557321 389 17 30.8/5.56 gi|2098257 355 8 13.9/5.35 gi|15277503 430 17 40.1/5.55 2.7 21.66 2.86 22.87 gi|48145933 121 4 gi|1620909 79 6 gi|223092 151 4 18.7/5.31 11.6/5.43 16.0/5.5 gi|178775 307 18 29.0/5.45 Thyroid hormone transport, serine proteinase inhibitors, acute-phase protein, tumorf 12.8/5.33 42.3/6.23 4 39.0/6.27 Affects the amyloid pathway Isoform of haptoglobin Recycling of heme- iron, acute-phase protein, Mucoproteins, tumorf 22.17 22.27 22.6 gi|1212947 289 gi|223976 318 6 gi|55669575 442ACTB proteinChain A, TTR VariantsApolipoprotein A-I preproproteinProapolipoproteinCeruloplasmin CpFCeruloplasminTransthyretinCovalent Dimer Of Transthyretin7 Biomarkers for Chemoresistant Ovarian CancerHaptoglobin HpHaptoglobinabSpot no. assigned by DeCyder 2-D Differential Analysis Software V6.0. corresponding to the DIGE image in Figure 1. Decreased or increased ratio of protein in ascites of intrinsic chemoresistant ovarian cancer compared to chemosensitive ones; “?’ indicates down-regulation of protein in ascites. Accession no. from NCBInr database. d Mascot scores greater than 40 1516647 were considered significant. e Protein function mainly categorized according to MeSH Tree Structure. f Alterations in the serum level may indicate: occurrence, tumor progression, response to therapy or prognosis for some malignancy reported in the literature. doi:10.1371/journal.pone.0051256.tcBiomarkers for Chemoresistant Ovarian CancerTable 3. Concentrations of proteins in ascites of intrinsically chemoresistant and chemosensitive EOC.Analytes Apo-AIV (mg/mL) CP (mg/mL) HB (mg/L) TTR (mg/L)Chemosensitive 230.3641.4 157.5611.5 263.1638.0 316.2635.Chemoresistant 215.0635.7 192.2619.5 266.6644.1 337.9636.P value0.349 0.001 0.832 0.*Apo-AIV, apoliprotein A-IV; CP, ceruloplasmin; HB, haptoglobin; TTR, transthyretin. doi:10.1371/journal.pone.0051256.tThe change in serum concentration of ceruloplasmin, along with many other acute phase proteins identified among the differentially expressed proteins, implicated t.
